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. 2020 Jun 6;2020:7865484. doi: 10.1155/2020/7865484

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Copyright © 2020 Sigrid Müller-Deubert et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PDE10A expression in hMSC after osteogenic differentiation. QPCR (a) and Western blot (b) analysis of PDE10A as well as the expression of the osteogenic markers RUNX2 and ALPL (c) in primary human MSC (n = 4) differentiated towards the osteogenic lineage for different time points as indicated. RPS27A was used as the housekeeping gene; β-actin was used as loading control. QPCR data were obtained from technical triplicates, and results are shown as mean ± SEM. Fold change was calculated with the ΔΔCT method. Significances were calculated with the Student t-test (^∗p < 0.05; ^∗∗p < 0.005). A representative Western blot is shown. ALPL: tissue nonspecific alkaline phosphatase; c: control; oD: osteogenic differentiation; PDE10a: phosphodiesterase 10a; RUNX2: runt-related transcription factor 2.