Figure 5.

Gene expression after cyclic stretching of primary MSCs derived from 13 donors. Relative mRNA expression of PDE10A and the mechanoresponsive genes FOS and PTGS2 15 min and 4 h after mechanical loading. QPCR data were obtained from three independent qPCR experiments. Results are shown as mean ± SEM; fold change was calculated with the ΔΔCT method and normalized to basal activity (nonstretched, dashed line). RPS27A served as the housekeeping gene. Significances were calculated with the Student t-test (^∗p < 0.05). FOS: fos protooncogene; PDE10A: phosphodiesterase 10A; PTGS2: prostaglandin-endoperoxide synthase 2.