Table 1.
Primer names, sequences, product lengths, annealing temperatures, and GenBank Accession numbers are shown.
| Gene name | Primer | Sequence 5′-3′ | Product length | Annealing temp (°C) | GenBank accession number |
|---|---|---|---|---|---|
| Primers for human genes | |||||
| ALPL | ALPL_FOR | GTACGAGCTGAACAGGAACAACG | 151 | 58 | NM_000478.5 |
| ALPL_REV | CTTGGCTTTTCCTTCATGGTG | ||||
| RPS27A [37] | RPS27A_FOR | TCGTGGTGGTGCTAAGAAAA | 141 | 60 | NM_001135592 |
| RPS27A_REV | TCTCGACGAAGGCGACT | ||||
| RUNX2 | Runx2_FOR | CTTCACAAATCCTCCCCAAG | 147 | 58 | NM_001024630.3 |
| Runx2_REV | ATGCGCCCTAAATCACTGAG | ||||
| SPP1 | SPP1_FOR | TATGATGGCCGAGGTGATAG | 133 | 60 | NM_001040058.2 |
| SPP1_REV | CATTCAACTCCTCGCTTTCC | ||||
| Primers obtained from Qiagen | |||||
| PDE10A | Hs_PDE10a_1_SG | Qiagen sequence | NM_001130690; NM_006661 | ||
| PTGS2 | Hs_PTGS2_1_SG | Qiagen sequence | 59 | NM_000963 | |
| FOS | Hs_FOS_1_SG | Qiagen sequence | 57 | NM_005252.3 | |
| Primers for murine genes | |||||
| B2m | mB2m_FOR | GTCTTTCTGGTGCTTGTCTC | 117 | 57 | NM_009735.3 |
| mB2m_REV | AGTTCAGTATGTTCGGCTTC | ||||
| Pde10a | mPDE10A_FOR | TCTGAAAGTGTTAGTGCAGAGA | 98 | 57 | NM_001290707.1 |
| mPDE10A_REV | TGGTACCTGCTGACTTCCTT | ||||
| mSPP1_REV | CGCTCTTCATGTGAGAGGTG | ||||