Figure 7.

Effect of CNO on stimulus investigation time and activity level. A, Time subject mice spent investigating different stimuli following administration of CNO or vehicle. CNO did not significantly affect the amount of time Amigo2-icreERT2+ or Amigo2-icreERT2– mice spent investigating animals or objects. B, Comparison of time spent investigating animals versus objects following either vehicle or CNO administration. Neither Amigo2-icreERT2+ nor Amigo2-icreERT2– mice exhibited a preference for one stimulus type over the other following vehicle or CNO administration. C, D, Mean distance traveled (C) and time spent mobile (D) measured across recording days for Amigo2-icreERT2+ (blue line) and Amigo2-icreERT2– (red line) mice. Mobile periods were based on a 7-cm/s movement threshold with speeds greater than the threshold considered periods of mobility. Days on which CNO was administered are indicated by hash-marked bars or boxes, and days on which vehicle was administered are indicated by solid white or color bars or boxes. Amigo2-icreERT2+ cohorts are represented by red lines and bars and Amigo2-icreERT– cohorts are represented by blue lines and bars. For A, B, data points represent individual subject values and bars represent group averages. Circles indicate when an animal was used as the stimulus and triangles indicate when the stimulus was an object. Error bars indicate the SEM.