Figure 5.

Intersection of somatic and muscarinic activation. A, Example trace of a cell that received somatic depolarization while oxo-m was in the bath. B, Time graph for changes in spiking relative to baseline, somatic, and oxo-m stimulation occurs at minute 5 to 10. C, Bar graph for change in spiking relative to baseline. Combined stimulation was significantly different from baseline (p = 4.9 × 10⁻⁴), and significantly different from somatic or oxo-m stimulation alone (p = 0.035 and p = 0.044, respectively). D, Diagram for how somatic depolarization and muscarinic pathways overlap and interact with SK2 channels. E, Difference in initial firing rate for somatic and synaptic induction protocols. Both groups increased their firing rate per sweep from baseline to post (p = 0.002 and p = 0.020, respectively). F, Spike attenuation ratios for somatic and synaptic induction protocols. The spike attenuation ratio is a ratio of the spiking that takes place in the first half of the sweep, and decreases for both somatic and synaptic cell groups (p = 0.045 and p = 0.034, respectively). G, Shift in attenuation ratio is strongly correlated to change in intrinsic excitability. All cells from groups which had ACSF in the bath during baseline and post are plotted, indicating a strong connection between firing later in sweeps and intrinsic plasticity (p = 3.8 × 10⁻⁴).