Figure 18.

Brainstem terminations of vagal Tac1-expressing afferents labeled by unilateral intraganglionic injection of AAV9-flex-GFP into Tac1-tdT. AAV-mediated GFP expression (green, enhanced by α-GFP immunoreactivity) is compared with ROSA26-mediated tdTomato expression (red, native) in coronal sections of the medulla. A, B, 3D reconstruction of medulla along entire rostral–caudal axis. A, Rostral aspect. B, Dorsal aspect. C–H, Coronal sections of medulla from rostral to caudal, with labeling for the position relative to obex. C, nTS at +320 μm (relative to obex). D, High-magnification image of area identified by white box in C. E, F, nTS at obex (E) and −320 μm (F). G, Pa5 at −320 μm. H, nTS at −640 μm. The following structures are identified: area postrema (AP), central canal (CC), DMX, Pa5, SolC (C), SolCe (Ce), SolDL (DL), SolG (G), SolIM (IM), SolM (M), SolV (V), SolVL (VL), and TS. Scale bars: A, B, 1 mm; C, E, G, 200 μm; D, 50 μm.