Figure 19.

Brainstem terminations of vagal TRPV1⁺ and TRPV1⁻ afferents labeled by unilateral intraganglionic injection of AAV9-flex-tdT and AAV9-GFP into TRPV1-Cre. Cre-sensitive AAV-mediated tdTomato expression (red; enhanced by α-DsRed immunoreactivity) is compared with constitutively active AAV-mediated GFP expression (green, enhanced by α-GFP immunoreactivity) and neurotrace (blue) in coronal sections of the medulla (labeling for the position relative to obex). A–D, nTS from rostral to caudal. E, High-magnification image of SolC, SolM, and AP from C. F, High-magnification image of SolV, SolVL, and TS from C. G, Pa5 at −120 μm. The following structures are identified: area postrema (AP), DMX, SolC (C), SolCe (Ce), SolDL (DL), SolG (G), SolIM (IM), SolM (M), SolV (V), SolVL (VL), and TS. Scale bars: A, B, C, G, 200 μm; D, 100 μm; E, F, 40 μm.