Figure 11.

Hemispheric bias for MECP2 expression in individual NH brains. (A–E, A′–E′) Representative 20X magnification, tiled projection epifluorescent images showing MECP2 expression in left (A–E) and right (A′–E′) SS1 of NH from cohorts 1-5. R = rostral, C = caudal, D = dorsal and V = ventral. (A′′–E′′) Percentage cumulative frequency distribution of MECP2 intensity within left (grey) and right (black) SS1 of the corresponding NH cohorts. Cohort 1 (A′′) expressed more MECP2 in the right hemisphere than the left. Cohorts 2, 4–5 (B′′, D′′–E′′, respectively) expressed more MECP2 in the left hemisphere than the right, while cohort 3 (C′′) showed similar MECP2 expression in both hemispheres. A.U. = arbitrary intensity unit. N = 1 image per hemisphere.