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. 2020 Jun 9;7(3):ENEURO.0500-19.2020. doi: 10.1523/ENEURO.0500-19.2020

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Copyright © 2020 Lau et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 12. — Hemispheric bias for MECP2 expression in individual SH brains. (A–E, A′-E′) Representative 20X magnification, tiled projection epifluorescent images showing MECP2 expression in left (A–E) and right (A′-E′) SS1 of SH from cohorts 1-5. R = rostral, C = caudal, D = dorsal and V = ventral. (A′′-E′′) Percentage cumulative frequency distribution of MECP2 intensity within left (grey) and right (black) SS1 of the corresponding SH cohorts. Cohort 1 and 3 (A′′ and C′′, respectively) expressed more MECP2 in the right hemisphere than the left. Cohorts 2 and 5 (B′′ and E′′, respectively) expressed slightly more MECP2 in the left hemisphere than the right, while cohort 4 (D′′) showed similar MECP2 expression in both hemispheres. A.U. = arbitrary intensity unit. N = 1 image per hemisphere.