Figure 14.

Hemispheric bias for MECP2 expression in individual SW brains. (A–E, A′–E′) Representative 20X magnification, tiled projection epifluorescent images showing MECP2 expression in left (A–E) and right (A′–E′) SS1 of SW from cohorts 1-5. R = rostral, C = caudal, D = dorsal and V = ventral. (A′′–E′′) Percentage cumulative frequency distribution of MECP2 intensity within left (grey) and right (black) SS1 of the corresponding SW cohorts. Cohort 1 and 2 (A′′ and B′′, respectively) expressed more MECP2 in the right hemisphere than the left. MECP2 expressions in Cohorts 3-5 (C′′-E′′) were similar between left and right hemispheres. A.U. = arbitrary intensity unit. N = 1 image per hemisphere.