Figure 3.

Box-size dependence of the binding probability p_b(V) is determined by B_ij and K_d via eq 15. We show simulation results (blue) for three protein pairs (top to bottom). Error bars indicate the blocked standard errors of the mean. The lines are predictions using eq 15 and estimates for K_d and B_ij obtained at a box volume = 3375 nm³ (magenta vertical line) using the insertion/removal method (black, solid lines) and the subvolume method (red, dashed lines).