. 2020 May 7;124(23):4673–4685. doi: 10.1021/acs.jpcb.9b11802

rem”) and the Subvolume Method (“subvol”)^a.

lysozyme	method	K_d [μM]	B₂₂ [nm³]	B₂₂^(b) [nm³]	B₂₂^(u) [nm³]
	ins/rem	5191 ± 63	–77 ± 4	–160 ± 2	83 ± 4
	subvol	5188 ± 68	–78 ± 4	–160 ± 2	82 ± 3

Ubi/CUE	method	K_d [μM]	B₂₃ [nm³]	B₂₃^(b) [nm³]	B₂₃^(u) [nm³]
	ins/rem	153 ± 1	–5444 ± 37	–5435 ± 37	–9 ± 3
	subvol	153 ± 1	–5455 ± 39	–5444 ± 38	–11 ± 3

UDG/Ugi	method	K_d [μM]	B₂₃ [nm³]	B₂₃^(b) [nm³]	B₂₃^(u)[nm³]
	ins/rem	0.25 ± 0.002	–3 332 000 ± 27 000	–3 332 000 ± 27 000	–94 ± 5
	subvol	0.25 ± 0.002	–3 308 000 ± 27 000	–3 308 000 ± 27 000	–81 ± 7

Errors are standard errors of the mean.

Table 1. Kd, Bij, and the Contributions of Binding Interactions, Bij(b), and Nonbinding Interactions, Bij, to Bij for Three Protein Complexes (PDB codes 6LYZ, 1OTR, 1UUG) for the Insertion/Removal Method (“ins/rem”) and the Subvolume Method (“subvol”)a.