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. 2020 Jun 10;13:5375–5386. doi: 10.2147/OTT.S242342

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© 2020 Deng et al.

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miR-124-3p.1 promotes carboplatin-induced apoptosis through the mitochondrial pathway. (A) After treatment with miR-124-3p.1, CAV1 plasmid and carboplatin (0.5 μM) in SKOV3 and A2780 cells, phosphorylation of AKT and Bad was evaluated by Western blot analysis. (B) Interaction with Bad and Bcl-xl was evaluated by immunoprecipitation and Western blot analysis. (C) Mitochondria was separated from cytoplasm by using mitochondria/cytosol fraction kit. Western blot analysis was performed to detect the Bax in the mitochondria fraction (mit) and cytosol fraction (cyto) respectively. (D) Interaction with Bax and Bak was evaluated by immunoprecipitation and Western blot analysis. (E) Mitochondria was separated from cytoplasm by using mitochondria/cytosol fraction kit. Western blot analysis was performed to detect the cytochrome c in the mitochondria fraction (mit) and cytosol fraction (cyto) respectively. (F) Cleavage of caspase-9 and caspase-3 was evaluated by Western blot analysis.

Abbreviations: miR-124-3p.1, microRNA-124-3p.1; CAV1, caveolin-1; AKT, threonine protein kinase; Bad, BCL2 associated agonist of cell death; Bcl-xl, B-cell lymphoma-extra large; Bax, BCL2 associated X; Bak, BCL2 antagonist/killer.