Figure 7.

The combined effects of ELE and 5-FU on NF-κB/COX-2 signaling pathway.

Notes: (A) MDA-Mb-231 and BT459 cells were treated with ELE alone, 5-FU alone, or their combination. We used Western blot to check the p65 and p50 expression levels in the nucleus from the nuclear lysate, p-P65, p65, p-IKKα/β, p-IκBα, and IκBα in the cytoplasmic lysate while detecting the COX-2 expression level from the whole cell lysate. We used GAPDH as the loading control for whole cell and cytoplasmic proteins, while Lamb1 was used for nuclear proteins. The data obtained from three independent experiments were presented as mean ± SD, and the significance levels were *P<0.05, **P<0.01, and ***P<0.001. (B) The subcellular localization of p65 and p50 was detected through immunofluorescence assay of BT549 cells after treatment with ELE alone, 5-FU alone, or their combination.