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. 2020 Jun 15;11:62. doi: 10.1186/s40104-020-00468-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Evaluation of antibacterial effect of TTO. (a) The growth inhibition of S. aureus during 24 h treatment with 0, 0.0125%, 0.025%, 0.05%, or 0.1% TTO (n = 3). Log CFU bacteria were measured after 3 h, 6 h, 12 h, and 24 h. Means at the same time point with different letters (a, b) differ significantly for treatment effect. (b) Biofilm formation. The S. aureus cultures were incubated with 0 (control group), 0.00625%, 0.0125%, 0.025%, 0.05%, or 0.1% TTO (n = 6) and absorbance at 540 nm measured. (c) The association of BMECs. The BMEC were incubated in DMEM/F12 medium containing S. aureus and 0 (control group), 0.025%, 0.05%, or 0.1% TTO (n = 3). (d) The invasion of BMECs. The BMEC were incubated in DMEM/F12 medium containing S. aureus and 0 (control group), 0.025%, 0.05%, or 0.1% TTO (n = 3). Data are presented as the means ± SEM. Means at the different concentration of TTO with different letters (a-c) differ significantly for treatment effect