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. 2020 Jun 15;11:62. doi: 10.1186/s40104-020-00468-9

Table 1.

Primers for real-time PCR analysesa

Gene Primer sequence (5′ to 3′) Accession number Size, bp
GAPDH F:GGGTCATCATCTCTGCACCT NM_001034034 176
R:GGTCATAAGTCCCTCCACGA
IL-6 F:TCCTTGCTGCTTTCACACTC NM_173923.2 129
R:CACCCCAGGCAGACTACTTC
IL-1β F:CAGTGCCTACGCACATGTCT NM_174093.1 209
R:AGAGGAGGTGGAGAGCCTTC
TNF-α F:GCCCTCTGGTTCAGACACTC NM_173966.3 192
R:AGATGAGGTAAAGCCCGTCA
TLR-2 F:CAGGCTTCTTCTCTGTCTTGT NM_174197.2 140
R:CTGTTGCCGACATAGGTGATA
IL-8 F:TGGGCCACACTGTGAAAAT NM_173925.2 136
R:TCATGGATCTTGCTTCTCAGC
κ-casein F: CCAGGAGCAAAACCAAGAAC NM_174294.2 148
R: TGCAACTGGTTTCTGTTGGT
IL-10 F:TGTATCCACTTGCCAACCAG NM_174088.1 126
R:CAGCAGAGACTGGGTCAACA
L-selectin F:CTCTGCTACACAGCTTCTTGTAAACC NM_174182.1 104
R:CCGTAGTACCCCAAATCACAGTT
IRAK1 F: CCTCAGCGACTGGACATCCT NM_001040555.1 103
R:GGACGTTGGAACTCTTGACATCT
NFKBIA F:GGGAGACCTGGCCTTCCTC NM 001045868.1 101
R:CCAGAAGTGCCTCAGCGATT
TRAF6 F:AGAACAGATGCCCAATCACTATGAT NM_001034661.2 100
R:GTGATTCCTCTGCATCTTTTCATG
Lysozyme F:GAGAGATGTGAGCTTGCCAGAA NM_001078159.1 120
R:TGTAGCTTGTGTGTTGTAATTGCTTTC
SOD2 F:GAGAAGGGTGATGTTACAGCTCAGA NM_201527.2 100
R:GGCTCAGATTTGTCCAGAAGATG
TLR-4 F:GACCCTTGCGTACAGGTTGT NM_174198.6 103
R:GGTCCAGCATCTTGGTTGAT
β-actin F:GACCCAGATCATGTTCGAGA NM_173979.3 145
R:CTCATAGATGGGCACCGTGT

aAbbreviations: GAPDH Glyceraldehyde-3-phosphate dehydrogenase, IRAK1 Interleukin 1 receptor associated kinase 1, NFKBIA NFKB inhibitor alpha, TRAF6 TNF receptor associated factor 6, SOD2 superoxide dismutase 2, TLR-2 Toll like receptor 2, TLR-4 Toll like receptor 4, F Forward; R Reverse