Figure 2.

In vitro screening of OR51E1. (a) Response of OR51E1 to six controls (three non-agonists and three agonists, in black), 4 predicted agonists by the SVM model (in red), 28 predicted non-agonists by the SVM model (in blue), and 44 molecules not within the applicability domain (in gray). Odorants were injected at 100 μM, and each response of OR51E1 was corrected by that of the empty vector (pCI) response and by basal activity of receptors. It should be noted that negative responses do not reflect inhibition of the response but rather cell toxicity. (b) Dose-response curves for the four potential agonists as identified by the SVM model. Cyclobutanecarboxylic acid and 2-methylbutyric acid were found to activate the receptor, while S-(+)-octanol and dimethyl trisulfide did not. All responses were normalized to that of nonanoic acid.