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. 2020 Apr 3;11(6):1269–1273. doi: 10.1021/acsmedchemlett.0c00111

Figure 5.

Figure 5

Mass spectrometry analysis reveals that TMX-1120 and TMX-2164 react with BCL6 protein at Tyr58. (A) Mass spectra (left) and zero-charge mass spectra (right) of BCL6 protein treated with DMSO (top) or 10-fold molar excess of TMX-1120 (bottom) for 2 h at room temperature. The observed mass shift of 429 Da is consistent with covalent addition of a single molecule of TMX-1120 (with loss of HF). (B) CE-MS/MS spectrum of tryptic BCL6 peptide (residues 48–66) with Y58 modified by TMX-1120. Ions of type b and y are indicated with blue and red glyphs, respectively. Y*, TMX-1120-modified tyrosine. Panels C and D are the same as panels A and B except with TMX-2164 (mass shift of 572 Da is consistent with TMX-2164 with loss of HF).