Table 1. Kinetics of Compound Binding to Naa50_apo and Naa50·AcCoA by SPRa.
| Protein | Compound | kon (M–1·s–1) | koff (s–1) | t1/2 (min) | SPR KD (nM) |
|---|---|---|---|---|---|
| Naa50_apo | AcCoA | 1.8 × 105 | 3.1 × 10–3 | 4 | 17.6 ± 2.8 |
| Naa50_apo | CoA | 2.0 × 105 | 3.2 × 10–2 | 0.4 | 156 ± 27 |
| Naa50_apo | 4a | 4.26 × 104 | 4.5 × 10–3 | 2.6 | 105 ± 4.8 |
| Naa50·AcCoA | 4a | 5.5 × 104 | 1.4 × 10–3 | 8.4 | 26.7 ± 7.3 |
| Naa50·AcCoA | 4b | 3.6 × 103 | 1.7 × 10–3 | 8.6 | 470 ± 25 |
| Naa50·AcCoA | 3a | 4.3 × 103 | 6.0 × 10–3 | 3.1 | 1010 ± 963 |
| Naa50·AcCoA | 3b | ND | ND | ND | >10,000 |
a
Measured at 10 °C in 25 mM HEPES, 150 mM NaCl, 0.5 mM TCEP, 5% glycerol, 0.02% Tween 20, 2% DMSO, pH 7.5.