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. 2020 May 20;19(6):e13157. doi: 10.1111/acel.13157

FIGURE 2.

FIGURE 2

HSF‐1 is required for heat shock and starvation‐induced stress granule formation in Caenorhabditis elegans. (a) tiar‐1::gfp transgenic animals were treated with L4440 or hsf‐1 RNAi from eggs. TIAR‐1::GFP foci in the intestinal cells was observed under normal conditions or heat stress. (b) gtbp‐1::gfp transgenic animals were treated with L4440 or hsf‐1 RNAi from eggs. GTBP‐1::GFP foci in the intestinal cells was analyzed with or without heat stress. (c) TIAR‐1::GFP in wild‐type and hsf‐1 overexpressing mutant backgrounds. (d) tiar‐1::gfp transgenic animals were treated with L4440 or hsf‐1 RNAi from eggs. TIAR‐1::GFP foci in the intestinal cells were observed under ad libitum conditions or 2‐hr starvation. (e) eat‐2 mutants carrying TIAR‐1::GFP were treated with L4440 or hsf‐1 RNAi from eggs. Right panels, quantitative analysis of stress granules for (a–e). Each data point represents the number of stress granules per intestinal cell obtained from one image. Data were analyzed by Student's t test or two‐way ANOVA. Levels of significance are shown as ***p < .001. NS, not significant. Scale bar: 20 µm