FIGURE 6.

Label‐free shotgun proteomics of glomeruli from YWT, OWT, and OTG mice (n = 4 per group) (a–c). (a) Glomeruli were collected using laser capture microdissection, processed for proteomics (Orbitrap Lumos), and analyzed using Student's t tests (with corrections) for OWT versus YWT (aging effect) and OTG versus OWT (transgenic effect). (b, c) Heatmaps show comparisons of protein abundance of OWT versus YWT and OTG versus OWT, respectively. Red indicates increases, and blue indicates decreases, of abundance. Proteins were ranked according to fold‐change with age for 77 proteins (see Table S1 for details), N = 4. (d) Targeted proteomics of tubules from YWT, OWT and OTG, which was focused on 139 selected proteins involved in glycolysis, Krebs cycle, mitochondrial respiratory complexes, fatty acid oxidation & biosynthesis, peroxisomes, antioxidants, and proteostasis (see Table S2 for details). In each heatmap, top row indicates OWT versus YWT (aging effect) and bottom row indicates OTG versus OWT (effect of GPX1 overexpression). *Substantial decrease in key enzymes of fatty acid oxidation with aging