FIGURE 5.

GILZ expression in myeloid cells from young and aged mice. (a, b) GILZ expression in PMs from young and aged mice was determined by flow cytometry. (a) Representative histogram. (b) Background‐subtracted GILZ median fluorescence intensity (MFI) (young: n = 8, aged: n = 10). (c) GILZ MFI in myeloid cells in the liver and lymphoid tissues of young mice (n = 10). ***p < .001 compared with MFI values for total CD45⁺ (liver) or total cells (spleen and lymph node), ^## p < .01, ^### p < .001 compared with all other subsets (ANOVA with Bonferroni's post hoc test. (d) GILZ expression in myeloid subsets of aged mice. The GILZ MFI of young cells was set as 100% for each subset (n = 8). *p < .05, **p < .01, ***p < .001 compared with the same subset in young animals by Mann–Whitney U test. Box plots show the 25–75th percentiles (box), mean (circle), median (line), and SD (whiskers). Mono: monocytes; Mo/Mac: monocytes and macrophages; cDC: classical dendritic cells; KC: Kupffer cells