. 2020 Jun 10;40(24):4622–4643. doi: 10.1523/JNEUROSCI.2339-19.2020

Table 3.

Microglia percentage of non-neuronal cells in different cortical sites^a

	Gray matter			White matter			Wilcoxon, I%O in gray versus white matter
	Range	Mean ± SE	I% versus N/mg, Spearman r, and p value	Range	Mean ± SE	% versus O/mg, Spearman r, and p value	Wilcoxon, I%O in gray versus white matter
All clades	[0.88–28.15%]	7.56 ± 0.11%	ρ = −0.4805	[1.09–16.73%]	5.86 ± 0.11%	ρ = −0.2613	p < 0.0001
All clades	[0.88–28.15%]	7.56 ± 0.11%	p < 0.0001	[1.09–16.73%]	5.86 ± 0.11%	p < 0.0001
Per clade
Afrotheria	[4.67–16.39%]	10.18 ± 0.20%	ρ = 0.1082	[2.64–10.12%]	5.67 ± 0.27%	ρ = 0.1285	p < 0.0001
Afrotheria	[4.67–16.39%]	10.18 ± 0.20%	p = 0.2393	[2.64–10.12%]	5.67 ± 0.27%	p = 0.3895
Artiodactyla	[4.02–17.76%]	9.82 ± 0.43%	ρ = 0.2185	[3.60–14.64%]	7.40 ± 0.31%	ρ = 0.2997	p < 0.0001
Artiodactyla	[4.02–17.76%]	9.82 ± 0.43%	p = 0.0994	[3.60–14.64%]	7.40 ± 0.31%	p = 0.0235
Carnivora	[2.69–15.95%]	9.02 ± 0.16%	ρ = −0.1793	[1.80–16.73%]	9.15 ± 0.24%	ρ = 0.2002	p = 0.6766
Carnivora	[2.69–15.95%]	9.02 ± 0.16%	p = 0.0069	[1.80–16.73%]	9.15 ± 0.24%	p = 0.0112
Marsupialia	[0.89–15.45%]	8.40 ± 0.27%	ρ = 0.0954	[1.26–12.18%]	5.53 ± 0.16%	ρ = −0.1915	p < 0.0001
Marsupialia	[0.89–15.45%]	8.40 ± 0.27%	p = 0.2521	[1.26–12.18%]	5.53 ± 0.16%	p = 0.0324
Primata	[0.88–28.15%]	5.44 ± 0.16%	ρ = −0.1184	[1.09–13.65%]	4.06 ± 0.11%	ρ = −0.0805	p < 0.0001
Primata	[0.88–28.15%]	5.44 ± 0.16%	p = 0.0148	[1.09–13.65%]	4.06 ± 0.11%	p = 0.1554
Per species
Afrotheria^*
L. africana	[4.67–14.73%]	10.12 ± 0.20%	ρ = 0.0834	[2.64–9.13%]	5.60 ± 0.27%	ρ = 0.1787	p < 0.0001
L. africana	[4.67–14.73%]	10.12 ± 0.20%	p = 0.3710	[2.64–9.13%]	5.60 ± 0.27%	p = 0.2458
Artiodactyla
G. camelopardalis	[6.81–17.76%]	12.47 ± 0.57%	ρ = −0.1083	[3.60–10.64%]	7.43 ± 0.42%	ρ = −0.1293	p < 0.0001
G. camelopardalis	[6.81–17.76%]	12.47 ± 0.57%	p = 0.6496	[3.60–10.64%]	7.43 ± 0.42%	p = 0.5869
T. strepsiceros	[4.91–9.79%]	7.06 ± 0.34%	ρ = 0.3147	[4.45–8.85%]	6.41 ± 0.30%	ρ = −0.3294	p = 0.1468
T. strepsiceros	[4.91–9.79%]	7.06 ± 0.34%	p = 0.2352	[4.45–8.85%]	6.41 ± 0.30%	p = 0.2128
S. scrofa domesticus	[9.18–15.19%]	12.25 ± 0.60%	ρ = −0.6121	[9.22–14.64%]	11.25 ± 0.63%	ρ = 0.0333	p = 0.3913
S. scrofa domesticus	[9.18–15.19%]	12.25 ± 0.60%	p = 0.0600	[9.22–14.64%]	11.25 ± 0.63%	p = 0.9322
A. marsupialis	[4.02–8.56%]	7.06 ± 0.41%	ρ = 0.4895	[3.82–6.96%]	5.79 ± 0.24%	ρ = 0.3636	p = 0.0166
A. marsupialis	[4.02–8.56%]	7.06 ± 0.41%	p = 0.1063	[3.82–6.96%]	5.79 ± 0.24%	p = 0.2453
Carnivora
M. mongo	[2.69–5.62%]	4.18 ± 0.41%	ρ = 0.5357	[1.80–5.60%]	3.93 ± 0.31%	ρ = −0.1748	p = 0.6420
M. mongo	[2.69–5.62%]	4.18 ± 0.41%	p = 0.2152	[1.80–5.60%]	3.93 ± 0.31%	p = 0.5868
U. arctos	[4.98–15.95%]	9.02 ± 0.28%	ρ = −0.0980	[5.13–13.93%]	8.40 ± 0.31%	ρ = 0.0486	p = 0.1126
U. arctos	[4.98–15.95%]	9.02 ± 0.28%	p = 0.4335	[5.13–13.93%]	8.40 ± 0.31%	p = 0.7403
F. catus	[4.52–10.35%]	7.44 ± 0.35%	ρ = 0.5275	[3.64–9.68%]	6.10 ± 0.40%	ρ = −0.1265	p = 0.0317
F. catus	[4.52–10.35%]	7.44 ± 0.35%	p = 0.0047	[3.64–9.68%]	6.10 ± 0.40%	p = 0.6407
C. familiaris	[4.77–15.05%]	9.24 ± 0.31%	ρ = −0.2504	[5.51–14.23%]	9.87 ± 0.32%	ρ = −0.2209	p = 0.1064
C. familiaris	[4.77–15.05%]	9.24 ± 0.31%	p = 0.0794	[5.51–14.23%]	9.87 ± 0.32%	p = 0.2166
M. putorius furo	[5.31–13.20%]	9.70 ± 0.64%	ρ = 0.3909	[6.76–16.73%]	9.42 ± 0.96%	ρ = −0.6667	p = 0.0946
M. putorius furo	[5.31–13.20%]	9.70 ± 0.64%	p = 0.2345	[6.76–16.73%]	9.42 ± 0.96%	p = 0.0499
P. leo	[5.88–13.74%]	9.91 ± 0.22%	ρ = −0.3891	[8.40–15.54%]	12.13 ± 0.30%	ρ = −0.5305	p < 0.0001
P. leo	[5.88–13.74%]	9.91 ± 0.22%	p = 0.0014	[8.40–15.54%]	12.13 ± 0.30%	p = 0.0004
Marsupialia
M. rufrogriseus	[5.31–13.38%]	10.08 ± 0.45%	ρ = −0.0870	[4.92–12.18%]	6.72 ± 0.35%	ρ = −0.3404	p < 0.0001
M. rufrogriseus	[5.31–13.38%]	10.08 ± 0.45%	p = 0.7076	[4.92–12.18%]	6.72 ± 0.35%	p = 0.1539
D. aurita	[6.68–15.45%]	11.24 ± 0.63%	ρ = −0.1909	[4.63–8.45%]	6.57 ± 0.37%	ρ = −0.7413	p < 0.0001
D. aurita	[6.68–15.45%]	11.24 ± 0.63%	p = 0.4479	[4.63–8.45%]	6.57 ± 0.37%	p = 0.0058
D. goodfellowi	[3.63–10.43%]	7.03 ± 0.36%	ρ = −0.0400	[2.94–8.18%]	6.07 ± 0.29%	ρ = −0.5727	p = 0.0670
D. goodfellowi	[3.63–10.43%]	7.03 ± 0.36%	p = 0.8528	[2.94–8.18%]	6.07 ± 0.29%	p = 0.0067
M. parma	[3.40–11.19%]	7.43 ± 0.47%	ρ = 0.1561	[3.20–6.51%]	4.84 ± 0.26%	ρ = −0.1893	p = 0.0001
M. parma	[3.40–11.19%]	7.43 ± 0.47%	p = 0.5233	[3.20–6.51%]	4.84 ± 0.26%	p = 0.4993
W. bicolor	[1.81–14.31%]	10.91 ± 0.63%	ρ = −0.4674	[1.86–10.05%]	6.25 ± 0.39%	ρ = −0.1922	p < 0.0001
W. bicolor	[1.81–14.31%]	10.91 ± 0.63%	p = 0.0245	[1.86–10.05%]	6.25 ± 0.39%	p = 0.4039
S. harrisii	[0.89–3.85%]	2.30 ± 0.25%	ρ = 0.4231	[1.26–4.38%]	2.72 ± 0.30%	ρ = −0.0699	p = 0.3412
S. harrisii	[0.89–3.85%]	2.30 ± 0.25%	p = 0.1497	[1.26–4.38%]	2.72 ± 0.30%	p = 0.8290
M. fuliginosus	[4.68–12.13%]	7.90 ± 0.39%	ρ = 0.3914	[2.44–6.07%]	4.84 ± 0.18%	ρ = −0.3038	p < 0.0001
M. fuliginosus	[4.68–12.13%]	7.90 ± 0.39%	p = 0.0395	[2.44–6.07%]	4.84 ± 0.18%	p = 0.1398
Primata
P. anubis cynocephalus	[1.98–10.77%]	5.33 ± 0.31%	ρ = −0.3339	[2.27–8.88%]	5.03 ± 0.22%	ρ = 0.0117	p = 0.9779
P. anubis cynocephalus	[1.98–10.77%]	5.33 ± 0.31%	p = 0.0127	[2.27–8.88%]	5.03 ± 0.22%	p = 0.9391
M. fascicularis	[1.29–11.00%]	5.45 ± 0.34%	ρ = 0.0935	[1.09–10.06%]	5.47 ± 0.38%	ρ = −0.4674	p = 0.8726
M. fascicularis	[1.29–11.00%]	5.45 ± 0.34%	p = 0.5712	[1.09–10.06%]	5.47 ± 0.38%	p = 0.0053
O. garnettii	[10.60–28.15%]	18.60 ± 1.59%	ρ = −0.4462	[4.88–13.65%]	8.04 ± 0.94%	ρ = 0.2000	p = 0.0003
O. garnettii	[10.60–28.15%]	18.60 ± 1.59%	p = 0.1098	[4.88–13.65%]	8.04 ± 0.94%	p = 0.6059
H. sapiens sapiens	[1.13–15.94%]	4.71 ± 0.15%	ρ = −0.3137	[1.11–8.86%]	2.98 ± 0.12%	ρ = 0.0787	p < 0.0001
H. sapiens sapiens	[1.13–15.94%]	4.71 ± 0.15%	p < 0.0001	[1.11–8.86%]	2.98 ± 0.12%	p = 0.4075
A. trivirgatus	[1.56–5.12%]	3.14 ± 0.23%	ρ = −0.1377	[1.70–3.24%]	2.42 ± 0.11%	ρ = −0.1476	p = 0.0503
A. trivirgatus	[1.56–5.12%]	3.14 ± 0.23%	p = 0.5116	[1.70–3.24%]	2.42 ± 0.11%	p = 0.5590
S. midas	[0.88–6.75%]	4.77 ± 0.36%	ρ = 0.1464p = 0.6025	[2.02–6.40%]	4.04 ± 0.37%	ρ = 0.1484	p = 0.0972
S. midas	[0.88–6.75%]	4.77 ± 0.36%	p = 0.6286	[2.02–6.40%]	4.04 ± 0.37%
M. nemestrina	[2.75–12.55%]	6.11 ± 0.30%	ρ = −0.1228	[1.92–8.40%]	4.84 ± 0.21%	ρ = 0.1164	p = 0.0048
M. nemestrina	[2.75–12.55%]	6.11 ± 0.30%	p = 0.3671	[1.92–8.40%]	4.84 ± 0.21%	p = 0.4357
C. apella	[2.31–10.75%]	5.07 ± 0.30%	ρ = −0.4000	[1.16–6.70%]	3.73 ± 0.25%	ρ = −0.2834	p = 0.0022
C. apella	[2.31–10.75%]	5.07 ± 0.30%	p = 0.0087	[1.16–6.70%]	3.73 ± 0.25%	p = 0.1043

^aI%O, Microglial percentage of non-neuronal cells; N/mg, number of neuronal cells per milligram of structure; O/mg, number of non-neuronal (other) cells per milligram of structure.

*For Afrotheria, analyses were done including all the species of the clade (D. dorsalis, E. myurus, L. africana, P. tetradactylus and P. capensis); Analyses within afrotherian species cortices were only possible where at least three data points were available (L. africana).