KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-Mouse NK1.1 | BioLegend | PK136 |
| Anti-Mouse CD3e | BioLegend | 17A2 |
| Anti-Mouse Ly6G | BioLegend | 1A8 |
| Anti-Mouse B220 | BioLegend | RA3-6B2 |
| Anti-Mouse IFN- γ | BioLegend | XMG1.2 |
| Anti-Mouse CD11b | BioLegend | M1/70 |
| Anti- Mouse CD27 | Biolegend | LG.3A10 |
| Anti-Mouse CD19 | BioLegend | 6D5 |
| Anti-Mouse CD49b/DX5 | BioLegend | DX5 |
| Anti-Mouse KLRG1 | BioLegend | 2F1 |
| Anti-Mouse CD45.1 | BioLegend | A20 |
| Anti-Mouse CD45.2 | BioLegend | 104 |
| Anti-Mouse CD8α | BioLegend | 53-6.7 |
| Anti-Mouse CD4 | BioLegend | GK1.5 |
| Anti-Mouse TCRβ | BioLegend | H57-597 |
| Anti-Mouse CD90.2 | BioLegend | 30-H12 |
| Anti-Mouse CD200r1 | BioLegend | OX-110 |
| Anti-Mouse CD11c | BioLegend | N418 |
| Anti-Mouse XCR1 | BioLegend | ZET |
| Anti-Mouse MHCII | BioLegend | M5/114.15.2 |
| Anti-Mouse CD64 | BioLegend | X54-5/7.1 |
| Anti-Mouse Ly49H | BioLegend | 3D10 |
| Anti-Cas9 | Cell Signaling Technology | 7A9-3A3 |
| Bacterial and Virus Strains | ||
| Murine Cytomegalovirus | O’Sullivan (PI) | Smith Strain |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Brefeldin A | BioLegend | Cat# 420601 |
| Monensin (Golgistop) | BioLegend | Cat# 420701 |
| Recombinant Mouse IL-12 | Peprotech | Cat# 200-12 |
| Recombinant Mouse IL-15 | Peprotech | Cat# 210-15 |
| 2′3′-cGAMP | InvivoGen | Cat# tlrl-nacga23 |
| Cas9-NLS | SYNTHEGO | N/A |
| Cas9-NLS | qb3 UC Berkley | N/A |
| Alt-R® Cas9 Electroporation Enhancer, 10 nmol | IDT | Cat# 1075916 |
| Synthetic Guide RNAs | SYNTHEGO | N/A |
| Critical Commercial Assays | ||
| DNeasy Blood & Tissue Kit | QIAGEN | Cat# 69504 |
| Cytofix/Cytoperm | BD Biosciences | Cat# 554714 |
| EasySep Mouse NK Cell Isolation Kit | Stem Cell | Cat# 19855 |
| EasySep Buffer | Stem Cell | Cat# 20104 |
| Experimental Models: Organisms/Strain | ||
| Mouse: C57BL/6 (CD45.2) | Jackson Lab | Stock # 000664 |
| Mouse: B6.SJL (CD45.1) | Jackson Lab | Stock #002114 |
| Mouse: Il12p40-YFP | Jackson Lab | Stock # 006412 |
| Mouse: Tmem173−/− | Jackson Lab | Stock # 017537 |
| Mouse: Stat4−/−, Klra8−/− (Ly49H-deficient), Xcr1Cre/+, Rosa26LsLDTA | O’Sullivan (PI) | N/A |
| Oligonucleotides | ||
| sgRNA and sgRNA PCR Primers see Table S1 | Multiple | N/A |
| Software and Algorithms | ||
| FlowJo, Version 9.9.6 | Ashland, OR: Becton, Dickinson and Company | https://www.flowjo.com/solutions/flowjo |
| Prism | GraphPad | http://www.graphpad.com/scientific-software/prism |
| ICE Analysis | SYNTHEGO | https://ice.synthego.com/ |
| Other | ||
| Neon Transfection System | ThermoFisher | Cat# MPK5000 |
| Neon 100 uL transfection kit | ThermoFisher | Cat# MPK10096 |