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. Author manuscript; available in PMC: 2020 Jun 15.
Published in final edited form as: Methods Mol Biol. 2020;2071:187–207. doi: 10.1007/978-1-4939-9857-9_11

Figure 1: Images of Toxoplasma gondii parasites expressing GCaMP6 in the cytoplasm, mitochondria and parasitophorous vacuole.

Figure 1:

A, Live cell imaging of extracellular tachyzoites of the RH strain expressing GCaMP6f (pTUBGCaMP6f) (green) in the cytosol and LAR-Geco (pDT7S4H3-SOD2-Mito-LAR-Geco) in the mitochondria (red). B, Live cell imaging of intracellular parasites expressing GCaMP6f (pTUBGCaMP6f) in the cytosol (green) and R-Geco (ptub_SAG1-IEα-R-GECO_dhfr_sag1CATsag1) in the PV (red). C, Live cell imaging of intracellular RH parasites expressing GCaMP6f (pDT7S4H3-P30-GCaMP6f) in PV.