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. 2020 Jul;98(1):24–37. doi: 10.1124/mol.120.119776

Fig. 2.

Fig. 2.

Cell proliferation assays in MCF-7:5C antihormone-resistant breast cancer cells. (A) Effects of test compounds alone after 7 days of treatment. Results show that the only two compounds able to completely inhibit the growth of the cells are E2 and Z2OHTPE, and the other two test compounds possess only minor inhibitory effects. Endoxifen was used as an antiestrogenic control and does not exhibit any biologic activity on the cells. (B) Antiestrogenic effects of test compounds in combination with 1 nM E2 after 7 days of treatment. Results show that all compounds, except Z2OHTPE, exhibit antiestrogenic effects after 7 days of treatment according to their intrinsic activity with the nonsteroidal antiestrogen endoxifen completely inhibiting the effect of E2. (C) Effects of test compounds alone on cells after 14 days of treatment. Results show that besides E2 and Z2OHTPE, the other two tested TPE derivatives BPTPE and 3OHTPE can inhibit the cell growth after 14 days of treatment. Endoxifen did not produce any inhibitory growth effect. All treatments were performed in triplicate; data represent the average of the replicates; error bars represent S.D.s with n = 3. Veh, vehicle.