Fig. 4.

Modulation of the transcriptional activity of pro-apoptotic genes. (A) Effects on BCL2L11 gene in MCF-7:5C cells after indicated durations with the indicated treatments. (B) Effects of the transcription of TP63 gene after same treatments. Results show that compounds BPTPE and 3OHTPE require longer treatments to induce higher levels of mRNA transcription of pro-apoptotic genes compared with E₂ after 72 hours of treatment, although still lower than E2. The only angular estrogen to induce apoptotic gene transcription at the same level after 72 hours of treatment as E2 is Z2OHTPE. All treatments were performed in triplicate; data represent the average of the replicates; error bars represent S.D.s with n = 3. Veh, vehicle.