Fig. 6.

Modulation of the transcriptional activity of estrogen-responsive genes. (A) Effects on well known estrogen-responsive gene TFF1 mRNA expression in MCF-7:5C cells after 24 hours of treatment with E₂ at 1 nM concentration and 1 µM for other test compounds. The results show that the tested compounds induce TFF1 gene mRNA expression partially and lower than the levels induced by E₂, all in concordance with their biologic activity. (B) GREB1 mRNA expression after 24 hours of treatment with E₂ at 1 nM concentration and 1 µM for other test compounds. The results are very similar to results seen in TFF1 gene regulation, with only Z2OHTPE being a full agonist. All treatments were performed in triplicate; data represent the average of the replicates; error bars represent S.D.s with n = 3.