Figure 2.

IL-1β stimulation promotes tumor growth in vitro and in vivo. SCC7 cells and B16-F10 cells were treated with 100 ng/ml IL-1β. Cell proliferation was evaluated using MTT assay. IL-1β stimulation enhanced viabilities of SCC7 cells (A) and B16-F10 cells (B). IL-1β significantly enhanced the colony formation capability of SCC7 cell C) and B16-F10 cells (D). Representative images of colony formation after IL-1β stimulation were demonstrated on the left. IL-1β stimulation could increase the tumorigenicity of SCC7 cells (E) and B16-F10 cells (F) in vivo. (*, P < 0.05; **, P < 0.01; ***, P < 0.001)