Figure 2.
Western-blot measured the expression of E-cadherin and other EMT related proteins (Vimentin, N-cadherin, and MMP2). Compared with the NICD over-expression group (group 2 vs group 1), the expression of Notch1, Snail, Vimentin, N-cadherin, MMP2 protein increased, and the expression of E-cadherin decreased. The expression of E-cadherin and other EMT related proteins was totally opposite in cells infected with Notch1-siRNA (group 4 vs group 3). (1 = pCDNA3.1(+), 2 = pCDNA3.1(+)-NICD, 3 = Notch1 ctr siRNA, 4 = Notch1 siRNA)