Figure 5.

Western blot measuring Notch1 and EMT related proteins expression after Snail interference. Western-blot showed that over-expression of Notch1 decreasing E-cadherin, caspase3, cleaved-caspase3, and increasing vimentin, N-cadherin, MMP2 and Bcl2 (group 1 vs group 2). However, siRNA mediated Notch 1 knock-down had the opposite effect (group 4 vs group 5). Snail would reverse the effect (group 2 vs group 3; group 5 vs group 6). (1 = Snail NC+pCDNA3.1(+)+Cisplatin, 2 = Snail NC + pCDNA3.1(+)-NICD + Cisplatin, 3=Snail siRNA+pCDNA3.1(+)-NICD+Cisplatin; 4= pCDNA3.1(+)+Notch1 NC Cisplatin; 5 = pCDNA3.1(+) + Notch1 siRNA + Cisplatin; 6 = pCDNA3.1(+)-Snail + Notch1 siRNA + Cisplatin)