Figure 8.

Ki67 staining analysis of cell proliferation after Snail interference. Over-expression of Notch1 promoted cell proliferation (group 1 vs group 2), while siRNA-mediated Notch1 knock-down inhibited cell proliferation (group 4 vs group 5), Snail would reverse the effect (group 2 vs group 3; group 5 vs group 6). Nuclear DNA was labelled in blue with DAPI. (1 = Snail NC + pCDNA3.1(+) + Cisplatin, 2 = Snail NC + pCDNA3.1(+)-NICD + Cisplatin, 3 = Snail siRNA+pCDNA3.1(+)-NICD + Cisplatin; 4 = pCDNA3.1(+) + Notch1 NC + Cisplatin; 5 = pCDNA3.1(+) + Notch1 siRNA + Cisplatin; 6 = pCDNA3.1(+)-Snail + Notch1 siRNA+ Cisplatin)