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. 2020 May 15;16(12):2063–2071. doi: 10.7150/ijbs.45999

Figure 7.

Figure 7

KLF10 is identified as a direct target of miR-106b-5p in MM. (A) miR-106b-5p and its putative binding sequence in the 3'-UTR of KLF10. The mutant binding site was generated in the complementary site for the seed region of miR-106b-5p. (B) miR-106b-5p was up-regulated in MM tissues compared to healthy donors. (C) miR-106-5p was knockdown by the miR-106b-5p inhibitors and measured by qRT-PCR. (D) qRT-PCR analysis of KLF10 mRNA expression in MM cells with anti-miR-106b-5p or anti-miR-NC vector transfection. (E) miR-106-5p knockdown increased the expression of KLF10 protein in MM cells. (F) miR-106b-5p significantly suppresses the luciferase activity that carried wild-type (wt) but not mutant (mt) 3'-UTR of KLF10. (G) A significant inverse correlation between the mRNA levels of KLF10 and miR-106b-5p was observed in MM tissues. n = six repeats with similar results. *P<0.05, n.s=no significance.