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. 2020 May 18;16(12):2159–2169. doi: 10.7150/ijbs.45933

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This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.

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Overexpression of miR-4516 inhibits pancreatic cell growth, migration and invasion and promotes cell apoptosis in pancreatic cells. PANC-1 or AsPC-1 cells were transfected with miR-4516 mimics or negative control miR-NC. (A) Cell viability was analyzed by CCK-8 assay at indicated time points. (B) DNA synthesis was analyzed by EdU/DAPI double staining. (C) Cell apoptosis was analyzed by Annexin V/PI double staining. (D) Cell migration and invasion was analyzed by transwell assay. *, P < 0.05; **, P < 0.01.