Figure 5.

OTX1 is highly expressed in pancreatic cancer tissues and knockdown of OTX1 suppresses pancreatic cell migration and invasion. (A) The protein expression of OTX1 in pancreatic cancer tissues (C) or adjacent non-tumor tissues (N) was analyzed by western blot. (B) The protein expression of OTX1 in pancreatic cancer cell lines (PANC-1, CAPAN-1, and AsPC-1) and control cell line HPDE6-C7 was analyzed by western blot. (C, D) PANC-1 or AsPC-1 cells were transfected with si-NC or si-OTX1. The relative expression of OTX1 mRNA or the protein expression of OTX1 was analyzed by qPCR and western blot 48 hours later. (E, F) Transwell assay was performed to evaluate the migration and invasion capability of PANC-1 or AsPC-1 cells transfected with si-NC or si-OTX1. (G, H) PANC-1 or AsPC-1 cells were transfected with si-NC or si-OTX1. The relative expression of MMP2 and MMP9 was analyzed by qPCR 48 hours later. *, P < 0.05