Skip to main content
. Author manuscript; available in PMC: 2021 May 14.
Published in final edited form as: Cell. 2020 Apr 15;181(4):848–864.e18. doi: 10.1016/j.cell.2020.03.047

Figure 5. Cluster 4 COPD clones are constitutively hyperinflammatory.

Figure 5.

A. Histogram depicting most significant (P<1.0e-8) pathways determined by Ingenuity Pathway Analysis of RNAseq differentially expressed genes (FDR<0.05) of patient-matched clones representative of Clusters 1–4. B. Differential expression heatmaps of chemokine, interleukin, and interferon genes among RNAseq DEGs (FDR<0.05) of patient-matched clones representative of Clusters 1–4 (SPN-13). C. H&E on sections through four-week xenografts of patient-matched clones of Clusters 1–4 showing that only Cluster 4 clones are accompanied by abundant intraluminal leukocytes. Scale bar, 100 μm. D. Immunofluorescence micrographs of Cluster 4 xenografts revealing high expression in epithelia of inflammatory mediators including IL33, CXCL8, and IL1B. Scale bar, 50 μm. E. CD45 immunohistochemistry of xenografts derived from Cluster 4 clone grown in vitro to passage 5 and to passage 25. Scale bar, 200 μm. F. Histogram of CXCL8, CCL20, and CXCL10 gene expression in clonal representatives of Clusters 1–4 at in vitro passage 5 and passage 25. See also Figure S5.