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. 2020 May 17;10(15):6615–6628. doi: 10.7150/thno.43169

Figure 6.

Figure 6

The number of mRNA-ITGA4 transfected (red boxes) and Control (violet boxes) hBM-MSCs migrating toward injured brain homogenate during 24 hours (transwell chambers assay). Homogenate from injured brain strongly repelled hBM-MSCs regardless of cell engineering or insert coating. The type III fixed effect test was used to determine statistical significance and the LMS method was used to compare. Box charts present the dispersion and shape of the data distribution for the test value in both tested cell populations. The length of the bars is equal to the quarter range (Q1-Q3) of the data, the tips of the moustaches indicate the minimum and maximum values, the line inside of the bar determines the median, while the square the arithmetic mean, the outliers are presented in the form of circles. *p<0.05, **p<0.01, ***p<0.001 (n=6). mRNA-ITGA4 - hBM-MSCs transfected with mRNA-ITGA4; Control - naive hBM-MSCs; Homogenate - homogenate of the brain tissue isolated from rats subjected to focal brain injury insult placed in the lower chamber of the transwell; VCAM1 - the membrane inserted between the upper and the lower transwell chambers, covered with VCAM1 protein.