Figure 4.

In vitro synergistic therapy against MDA-MB-231 cells. (A) Cell viability of MDA-MB-231 cells after treated with DoxNPs or DFTNPs at various concentrations after 36 h incubation. (B) Cell viability of MDA-MB-231 cells treated with or without DoxNPs at various concentrations of Fe³⁺/TA. (C) Intracellular ATP content treated with various concentrations of Fe³⁺/TA after standardization with cell number. (D) Cell viabilities after exposure to 808 nm laser irradiation under different concentrations. (E) Flow cytometry analysis of apoptosis after various treatments. (F) CLSM images of Calcein-AM and PI costained MDA-MB-231 cells after coincubation with DFTNPs for 4 h followed by various treatments. The scale bars are 50 μm.