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Representative confocal images of HCC70 cells incubated with HL647-hTAB004 at 4 °C and 37 °C at different time points (0, 20, 40, 60 min). Blue = DAPI, Green = WGA/cell membrane, Red = HL647-hTAB004. Scale bar, 30 µm. Confocal images for the 4 °C incubation over 60 min shows the HL647-hTAB004 (red) colocalized at the cell membrane (green). In contrast, confocal images for 37 °C over 60 min show the hTAB004 (red) being trafficked intracellularly.
