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. 2020 May 17;10(15):6638–6660. doi: 10.7150/thno.44793

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Genetic activation of Hh signaling by Sufu ablation suppressed expression of osteoclast-specific genes in vitro. qPCR analysis of relative mRNA levels of osteoclast-specific genes, including Nfatc1 (A), c-Fos (B), Acp5 (C), Oscar (D), Ctsk (E), Dcstamp (F), Atp6v0a3 (G), and Atp6v0d2 (H), in BMMs isolated from control and Sufu^CKO mice at 6 days after RANKL stimulation. Expression of each target gene was normalized by 18S ribosomal RNA. The relative changes in mRNA level were analyzed by 2^-ΔΔCT method. All values were calculated from three independent biological replicates and presented as mean ± SD. **P<0.01, compared with control group.