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. 2020 May 17;10(15):6638–6660. doi: 10.7150/thno.44793

Figure 4.

Figure 4

Genetic activation of Hh signaling by LysM-Cre-mediated deletion of Sufu gene prevented Ti particle-induced bone loss and suppressed osteoclastogenesis in vivo. (A) Representative 2D (bottom panels) and 3D (top panels) μCT images of calvariae from sham-operated control mice (Sham-Ctrl), SufuCKO mice with sham operation (Sham-SufuCKO), Ti particle-implanted control mice (Ti-Ctrl), and Ti particle-implanted SufuCKO mice (Ti-SufuCKO). (B-D) Quantitative μCT analyses of bone architecture parameters including bone volume/total tissue volume (BV/TV) (B), number of pores (C) and percentage of porosity (D). (E) Representative images of H&E staining of calvarial sections from Sham-Ctrl, Sham-SufuCKO, Ti-Ctrl, and Ti-SufuCKO mice. Scale bar, 100 μm. (F) Quantification of eroded surface proportion on H&E-stained calvarial sections. (G) Representative images of TRAP staining of calvarial sections from Sham-Ctrl, Sham-SufuCKO, Ti-Ctrl, and Ti-SufuCKO mice. Scale bar, 100 μm. (H) Quantitative analyses of number of TRAP-positive multinucleated cells. All bar graphs were presented as mean ± SD. n=5 mice per group, **P<0.01, determined by statistical analyses between two indicated groups. Sham-Ctrl, sham-operated control mice; Sham-SufuCKO, SufuCKO mice with sham operation; Ti-Ctrl, Ti particle-implanted control mice; Ti-SufuCKO, Ti particle-implanted SufuCKO mice.