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. 2020 May 17;10(15):6638–6660. doi: 10.7150/thno.44793

Figure 9.

Figure 9

Activation of Hh signaling did not affect RANKL-induced activation of AKT and NF-κB pathways. (A) Western blot analyses of protein levels of phosphorylated AKT (p-AKT), total AKT, phosphorylated NF-κB p65 (p-p65), total NF-κB p65 (p65), and GAPDH in BMMs treated with 50 ng/ml RANKL for indicate times in the presence of vehicle (Veh) or 2 µM PM (PM). Representative images from three independent biological replicates were shown. (B-C) Quantitative analyses of relative ratios of p-AKT/AKT (B) and p-p65/p65 (C). n=3 per group. (D) Western blot analyses of protein levels of NF-κB p65 (p65) in the cytoplasmic and nuclear extracts from BMMs treated with 50 ng/ml RANKL in the presence of vehicle (Veh) or 2 µM PM (PM) for indicate times. Representative images from three independent biological replicates were shown. (E-F) Quantitative analyses of relative ratios of cytoplasmic p65/GAPDH (E), and nuclear p65/PCNA (F). n=3 per group. All bar graphs were presented as mean ± SD.