Profile of circRNAs in ischemia and control muscle. (A) RNA-seq analyses of circRNA from ischemic and normal muscles. RPKM distribution showed good repeat among 3 samples. (B) Reads distribution of circRNA in genome. The circRNAs identified from exonic, intronic, and unknown were shown in “Purple”, “Blue” and “Brown”, respectively. (C) The quantity of circRNAs derived from different chromosomes. The circRNAs identified in control and ischemic samples are shown in “Red” and “Green”, respectively. (D) Venn diagram of circRNAs expression in ischemia and control groups. (E) circRNA-seq analyses showed the Read number in skeletal muscle. Read > 9 indicated higher expression. (F) RT-PCR analysis of some circRNA expression in normal mouse muscle.