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. 2020 May 18;10(15):6728–6742. doi: 10.7150/thno.42259

Figure 5.

Figure 5

circHIPK3 from UMSC-EXo inhibits inflammasome induced pyroptosis in skeletal muscle. (A) RT-PCR analysis of IL-1β and IL-18 mRNA expression in control and ischemic muscle. (B) ELISA analysis of serum IL-1β and IL-18 levels from control and ischemia groups. (C) RT-PCR analysis of IL-1β and IL-18 mRNA expression in PBS, Exo, and Exo-si-circHIPK3 groups. (D) ELISA analysis of serum IL-1β and IL-18 levels in the PBS, Exo, and Exo-si-circHIPK3 groups. (E) RT-PCR analysis showed increased NLRP3 mRNA expression in muscle after ischemic surgery. (F, G) Western blot analysis showed increased NLRP3 and Casp1 protein expression in muscle after ischemic surgery. (H) The mRNA expression of NLRP3 in muscle was decreased in the treatment of the Exo. (I, J) Expression of NLRP3 and Casp1 proteins in the PBS, Exo and Exo- si-circHIPK3 treated muscles. Data is shown as mean ± standard deviation (n=3). *P <0.05, **P <0.01, ***P <0.001.