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. 2020 May 25;10(15):6928–6945. doi: 10.7150/thno.43811

Figure 5.

Figure 5

FOXK1 is the downstream targeting molecule of SOX8. (A) GSEA analysis was performed using SOX8 knockdown cell line and the control (SKOV3-CisR/SOX8-i1 and SKOV3-CisR/Con). The signature was defined by genes with significant expression changes. (B and C) Immunoblotting and immunofluorescence assay detected the relation of SOX8 and FOXK1 in ovarian cancer cells. (D) FOXK1 mRNA expression level in Aurora-A knockdown ovarian cancer cell lines and SOX8 transfected cell lines in rescue experiment. (E) FOXK1 promoter activity affected by Aurora-A knockdown and SOX8 overexpression. (F) FOXK1 promoter activity affected by SOX8 silencing. (G) The region of SOX8 binding sites within the FOXK1 promoter. (H) ChIP results of the binding of SOX8 to the promoter of FOXK1. (I) The map of SOX8 binding sits in the promoter region of FOXK1. (J) Luciferase reporter assay was used for the detection of mutant sites in the promoter region of FOXK1. **P < 0.01.