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. Author manuscript; available in PMC: 2021 Jun 3.
Published in final edited form as: J Am Chem Soc. 2020 May 21;142(22):10042–10049. doi: 10.1021/jacs.0c01978

Figure 2.

Figure 2.

Mechanical unfolding of the bcl-2 hairpin (HP) within DNA nanocage (NC). (A) The DNA construct in Figure 1 is tethered between two optically trapped beads via affinity interactions. Inset shows unfolding and refolding transitions of the hairpin inside nanocage (HP@NC). Strep, Dig and Anti-Dig represent streptavidin, digoxigenin and anti-digoxigenin respectively. Force versus extension curve of the DNA hairpin (B) within and (C) without 15×15 nm nanocage. Red and black traces indicate stretching and relaxing curves, respectively. Zigzag features in insets depict rapid unfolding/refolding transitions. Experiments were performed in a 20 mM Tris buffer (pH 7.8) supplemented with 100 mM KCl, 10 mM MgCl2, and 1 mM EDTA at 25 °C.