Table I.
JS-K increases the formation of acidic vesicular organelles in breast cancer cells but not in non-transformed or normal mammary epithelial cells.
| Cells | JS-K (μM) | Acridine orange-stained positive cells (%) |
|---|---|---|
| MCF-7 | 0 | 4.7 |
| 1 | 19.3 | |
| 5 | 29.2 | |
| MDA-MB-231a | 0 | 3.1 |
| 1 | 13.6 | |
| 5 | 39.6 | |
| SKBr-3a | 0 | 4.8 |
| 1 | 29.7 | |
| 5 | 45.8 | |
| MDA-MB-453a | 0 | 4.4 |
| 1 | 17.9 | |
| 5 | 41.1 | |
| MDA-MB-468b | 0 | 2.6 |
| 5 | 18.4 | |
| HMECb | 0 | 2.8 |
| 5 | 4.1 | |
| MCF-10Ac | 0 | 7.1 |
| 5 | 9.3 | |
| Cells | JS-43-126 (μM) | Acridine orange-stained positive cells (%) |
| MDA-MB-231a | 0 | 3.8 |
| 5 | 3.5 | |
| MDA-MB-453a | 0 | 2.2 |
| 5 | 4.5 |
Breast cancer cells, plated at 1×105 cells per well in 6-well plates in 2 ml of DMEM/F12 medium containing 5% FBS, were treated with JS-K for 3 days and stained with acridine orange. The red fluorescence in the acridine orange-stained cells was detected by flow cytometry.
MDA-MB-468 and HMEC cells, plated at 4×105 cells per well in 6-well plates in 2 ml of HMEC medium containing bovine pituitary extract, were treated with JS-K for 3 days and stained with acridine orange. The red fluorescence in the acridine orange-stained cells was detected by flow cytometry.
MCF-10A cells, plated at 4×105 cells per well in 6-well plates in 2 ml of DMEM/F12 medium containing 10% FBS, 20 ng/ml epidermal growth factor, 20 ng/ml insulin-like growth factor-I, and 500 ng/ml hydrocortisone, were treated with JS-K for 3 days and stained with acridine orange. The red fluorescence in the acridine orange-stained cells was detected by flow cytometry.