Figure 4.
BCG Vaccination Elicits Myeloid Cell Fate Priming
(A) GOEA of upregulated genes in HSPCs. Top ten enriched terms (designated to letters “a” to “j”) are listed (BCG, D0, n = 14 versus D90, n = 15).
(B) Network representation of GO terms significantly enriched in upregulated DEGs in HSPCs (BCG, D0 versus D90, p < 0.01). Node size and color indicate contained gene numbers and p value for term nodes, respectively. Position and edges visualize relation of nodes to each other based on involved gene lists. Annotation of nodes by lowercase letters corresponds to (A).
(C) Unified genes from neutrophil pathways-associated genes from terms (ontology terms a–e, as indicated in A) (GO:0036230, GO:0002446, GO:0002283, GO:0043312, and GO:0042119) were extracted to generate an expression heatmap of HSPC transcriptomes.
(D) Expression heatmap of genes overlapping upregulated DEGs in HSPCs and genes associated with the term for the molecular function of receptor activity (GO: 0004872).
(E) Neutrophil counts (×109/L) of infants in the MIS BAIR study (BCG-vaccinated, n=385; BCG-naive, n = 409; ∗p < 0.05).
See also Tables S4–S7.