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. 2020 May 29;9:e57799. doi: 10.7554/eLife.57799

Figure 3. Poly-A tracts are correctly translated in P. falciparum cells.

Figure 3.

(A) 2D gel analysis of HA-IP samples of wild type reporter (-polyA36). The western blots show isoelectric point (pI) at 6.25 and 6.5 (arrows). (B) 2D gel of reporter with polyA tract (+polyA36) sample indicates pI 7.25 and 7.4 (arrows).( C) 2D gel analyses of reporter with twelve lysines coded by AAG codons (12 x AAG) indicates the same effect on pI value, pI is 7.25 and 7.4 (arrows). Overlay of images of PVDF membranes stained with Coomassie Brilliant Blue, and images of western blots probed with HA-antibody. The scale with pH is on the bottom. Coomassie-stained membranes show markers (right side) and tropomyosin (33 kDa) and pI at 5.2 (arrow) as an internal standard for Isoelectric focusing (IEF).