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. 2020 Jun 15;40(6):BSR20200740. doi: 10.1042/BSR20200740

Figure 3. HULC knockdown could rescue the inhibitory effect of LPS on progression of HUVECs.

Figure 3

HUVECs were treated with LPS for 48 h, and cells in control group were not given any treatment and after LPS-treated HUVECs were transfected with si-HULC or si-NC. (A) HULC expression was measured by qRT-PCR. (B,C) MTT and Flow cytometry assays were performed to detect cell viability and apoptosis, respectively. (D,E) The levels of Bcl-2, Bax, cleaved-caspase3, TNFα, IL-6, IL-8 and IL-1β were determined by western blot. (F–H) ROS, SOD and MDA productions were examined by DCFH-DA method and a commercial kit. *P<0.05.